Department of Molecular Genetics Kyoto University Graduate School of Medicine

Publications

• Mori, Y., Takashima, S., Kanatsu-Shinohara, M., Yi, Z., and Shinohara, T.
  Cell Rep36, 109550.
• Yamamuro, T., Nakamura, S., Yamano, Y., Endo, T., Yanagawa, K., Tokumura, A., Matsumura, T., Kobayashi, K., Mori, H., Enokidani, Y., Yoshida, G., Imoto, H., Kawabata, T., Hamasaki, M., Kuma, A., Kuribayashi, S., Takezawa, K., Okada, Y., Ozawa, M., Fukuhara, S., Shinohara, T., Ikawa, M., and Yoshimori, T.
  PLoS Genet.17, e1009688.
• Morimoto, H., Ogonuki, N., Kanatsu-Shinohara, M., Matoba, S., Ogura, A., and Shinohara, T.
  Stem Cell Reports 16, 1832-1844.
• Mori, Y., Ogonuki, N., Hasegawa, A., Kanatsu-Shinohara, M., Ogura, A., Wang, Y., McCarrey, J. R., and Shinohara, T.
  Biol. Reprod. 104, 706-716.
• Morimoto, H., Yamamoto, T., Miyazaki, T., Ogonuki, N., Ogura, A., Tanaka, T., Kanatsu-Shinohara, M., Yabe-Nishimura, C., Zhang, H., Pommier, Y., Trumpp, A., and Shinohara, T.
  Genes Dev.35, 250-260.
• Tanaka, T., Kanatsu-Shinohara, M., Lei, Z., Rao, C. V., Shinohara, T. The luteinizing hormone-testosterone pathway regulates mouse spermatogonial stem cell self-renewal by suppressing WNT5A expression in Sertoli cells.
  Stem Cell Reports 7:279-291 (2016)
• Kanatsu-Shinohara, M., Naoki, H., Shinohara, T. Nonrandom germline transmission of mouse spermatogonial stem cells.
  Dev. Cell 38: 248-261 (2016)
• Kanatsu-Shinohara, M., Morimoto, H., Shinohara, T. Fertility of male germline stem cells following spermatogonial transplantation in infertile mouse models.
  Biol. Reprod. 94:112 (2016)
• Kanatsu-Shinohara, M., Morimoto, H., Shinohara, T. Enrichment of mouse spermatogonial stem cells by the stem cell dye CDy1.
  Biol. Reprod. 94:13 (2016)
• Tanaka, T., Kanatsu-Shinohara, M., Hirose, M., Ogura, A., Shinohara, T. 2015. Pluripotent cell derivation from male germline cells by suppression of Dmrt1 and Trp53.
  J. Reprod. Dev. 61:473-484 (2015)
• Tanaka, T., Kanatsu-Shinohara, M., Shinohara, T. The CDKN1B-RB1-E2F1 pathway protects mouse spermatogonial stem cells from genomic damage.
  J. Reprod. Dev. 61:305-316 (2015)
• Morimoto, H., Kanatsu-Shinohara, M., Shinohara, T. ROS-generating oxidese Nox3 regulates the self-renewal of mouse spermatogonial stem cells. Biol. Reprod. 92:147 (2015)
• Takashima, S., Kanatsu-Shinohara, M., Tanaka, T., Morimoto, H., Inoue, K., Ogonuki, N., Jijiwa, M., Takahashi, M., Ogura, A., Shinohara, T. Functional differences between GDNF-dependent and FGF2-dependent mouse spermatogonial stem cell self-renewal.
  Stem Cell Reports 4: 489-502 (2015)
• Ishii, K., Ishiai, M., Morimoto, H., Kanatsu-Shinohara, M., Niwa, O., Takata, M., Shinohara, T. The Trp53-Trp53inp1-Tnfrsf10b pathway regulates the radiation response of mouse spermatogonial stem cells.
  Stem Cell Reports 3:676-689 (2014)
• Kanatsu-Shinohara, M., Ogonuki, N., Matoba, S., Morimoto, H., Ogura, A. Shinohara, T. Improved serum- and feeder-free culture of mouse germline stem cells.
  Biol. Reprod. 91:88 (2014)
• Kanatsu-Shinohara, M., Onoyama, I., Nakayama, K. I., Shinohara, T. Skp1-Cullin-F-box (SCF)-type ubiquitin ligase FBXW7 negatively regulates spermatogonial stem cell self-renewal.
  Proc. Natl. Acad. Sci. USA 111: 8826-8831 (2014)
• Kimura, T., Kaga Y., Ohta, H., Odamoto, M., Sekita, Y., Li, K., Yamano, N., Fujikawa, K., Isotani, A., Sasaki, N., Toyoda, M., Hayashi, K., Okabe, M., Shinohara, T., Saitou, M. and Nakano, T. Induction of primordial germ cell-like cells from mouse embryonic stem cells by EKR signal inhibition.
  Stem Cells 32:2668-2678 (2014)
• Ishii, K., Kanatsu-Shinohara, M., Shinohara, T. Cell-cycle-dependent colonization of mouse spermatogonial stem cells after transplantation into seminiferous tubules.
  J. Reprod. Dev. 60: 37-46 (2014)
• Kanatsu-Shinohara, M., Mori, Y., Shinohara, T. Enrichment of mouse spermatogonial stem cells based on aldehyde dehydrogenase activity.
  Biol. Reprod. 89:140 (2013)
• Kanatsu-Shinohara, M., Shinohara, T. Spermatogonial stem cell self-renewal and development.
  Annu. Rev. Cell Dev. Biol. 29: 163-187 (2013)
• Behringer, R. R., Shinohara, T. Symposium in honor of Ralph L. Brinster celebrating 50 years of scientific breakthroughs.
  Int. J. Dev. Biol. 57: 333-339 (2013)
• Takashima, S., Hirose, M., Ogonuki, N., Ebisuya, M., Inoue, K., Kanatsu-Shinohara, M., Tanaka, T., Nishida, E., Ogura, A., Shinohara, T. Regulation of pluripotency in male germline stem cells by Dmrt1.
  Genes Dev. 27:1949-1958 (2013)
• Morimoto, H., Iwata, K., Ogonuki, N., Inoue, K., Ogura, A., Kanatsu-Shinohara, M., Morimoto, T., Yabe-Nishimura, C., Shinohara, T. ROS are required for mouse spermatogonial stem cell self-renewal.
  Cell Stem Cell 12:774-786 (2013)
• Kanatsu-Shinohara, M., Morimoto, H., Shinohara, T. Enrichment of mouse spermatogonial stem cells by melanoma cell adhesion molecule expression.
  Biol. Reprod. 87:139 (2012)
• Kanatsu-Shinohara M., Inoue K., Takashima S., Takehashi M., Ogonuki N.,Morimoto H., Nagasawa T., Ogura A., Shinohara T. Reconstitution of mouse spermatogonial stem cell niches in culture.
  Cell Stem Cell 11:567-578 (2012)
  
• Ishii K., Kanatsu-Shinohara M., Toyokuni S., Shinohara T. FGF2 mediates mouse spermatogonial stem cell self-renewal via upregulation of Etv5 and Bcl6b through MAPK2K1 activation.
  Development 139(10):1734-1743 (2012)
  
• Takehashi M., Tada M., Kanatsu-Shinohara M., Morimoto H., Kazuki Y.,Oshimura M., Tada T., *Shinohara T. Hybridization of testis-derived stem cells with somatic cells and embryonic stem cells in mice.
  Biol. Reprod. 86(6):178 (2012)
  
• Morimoto H., Lee J., Tanaka T., Ishii K., Toyokuni S., Kanatsu-Shinohara M.,Shinohara T. In vitro transformation of mouse testis cells by oncogene transfection.
  Biol. Reprod. 86(5):148,1-11 (2012)
  
• Takashima S., Kanatsu-Shinohara M., Tanaka T., Takehashi M., Morimoto H.,Shinohara T. Rac mediates mouse spermatogonial stem cell homing to germline nishes by regulating transmigration through the blood-testis barrier.
  Cell Stem Cell 9(5):463-475 (2011)
  
• Kanatsu-Shinohara M., Takashima S., Ishii K., Shinohara T. Dynamic changes in EPCAM expression during spermatogonial stem cell differentiation in themouse testis.
  PLoS One. 6(8):e23663 (2011)
  
• Kanatsu-Shinohara M., Kato-Itoh M., Ikawa M., Takehashi M., Sanbo M., Morioka Y., Tanaka T., Morimoto H., Hirabayashi M., Shinohara T. Homologous recombination in rat germline stem cells.
  Biol. Reprod. 85(1):208-217 (2011)
• Shinohara T., Ishii K., Kanatsu-Shinohara M. Unstable side population phenotype of mouse spermatogonial stem cells in vitro.
  J. Reprod. Dev. 57(2):288-295 (2011)
• Kanatsu-Shinohara M., Inoue K., Ogonuki N., Morimoto H., Ogura A., Shinohara T. Serum- and feeder-free culture of mouse germline stem cells.
  Biol. Reprod. 84(1):97-105 (2011)
• Kanatsu-Shinohara M., Shinohara T. Germline modification using mouse spermatogonial stem cells.
  Methods Enzymol. 477:17-36 (2010)
• Kanatsu-Shinohara M., Takashima S., Shinohara T. Transmission distortion　by loss of p21 or p27 cyclin-dependent kinase inhibitors following competitive spermatogonial transplantation.
  Proc. Natl. Acad. Sci. USA 107(14):6210-6215 (2010)
• Takehashi M., Kanatsu-Shinohara M., Shinohara T. Generation of genetically modified animals using spermatogonial stem cells.
  Dev.Growth Differ. 52(3):303-310 (2010)
• Morimoto H., Kanatsu-Shionohara M., Takashima S., Chuma S., Nakatsuji N., Shinohara T. Phenotypic plasticity of mouse spermatogonial stem cells.
  PLoS One Nov 19;4(11):e7909 (2009)
• Kanatsu-Shinohara M., Ogonuki N., Miki H., Inoue K., Morimoto H., Takashima S., Ogura A., Shinohara T. Genetic influences in mouse spermatogonial stem cell self-renewal.
  J. Reprod. Dev. 56(1):145-153 (2010)
• Yoshimoto M., Heike T., Chang H., Kanatsu-Shinohara M., Baba S., Varnau J.T., Shinohara T., Yoder M,C., Nakahata T. Bone marrow engraftment but limited expansion of hematopoietic cells from multipotent germline stem cells derived from neonatal mouse testis.
  Exp. Hematol. 37(12):1400-1410 (2009)
• Lee J., Kanatsu-Shinohara M., Morimoto H., Kazuki Y., Takashima S., Oshimura M., Toyokuni S., Shinohara T. Genetic reconstitution of mouse spermatogonial stem cell self-renewal in vitro by Ras/cyclin D2 activation.
  Cell Stem Cell 5(1):76-86 (2009)
• Honda A., Hirose M., Inoue K., Hiura H., Miki H., Ogonuki N., Sugimoto M., Abe K., Kanatsu-Shinohara M., Kono T., Shinohara T., Ogura A. Large-scale production of growing oocytes in vitro from neonatal mouse ovaries.
  Int. J. Dev. Biol. 53(4):605-613 (2009)
• Takashima S., Takehashi M., Lee J., Chuma S., Okano M., Hata K., Suetake I., Nakatsuji N., Miyoshi H., Tajima S., Tanaka Y., Toyokuni S., Sasaki H., Kanatsu-Shinohara M., Shinohara T. Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects.
  Biol. Reprod. 81(1):155-164 (2009)
• Kanatsu-Shinohara M., Takehashi M., Shinohara T.
  Brief history, pitfalls, and prospects of mammalian spermatogonial stem cell research.
  Cold Spring Harb. Symp. Quant. Biol. 73:17-23 (2008)
• Lee J., Kanatsu-Shinohara M., Ogonuki N., Miki H., Inoue K., Morimoto H., Ogura A., Shinohara T. Heritable imprinting defect caused by epigenetic abnormalities in mouse spermatogonial stem cells.
  Biol. Reprod. 80(3):518-527 (2009)


Goto Top


• Kanatsu-Shinohara M., Takehashi M., Takashima S., Lee J., Morimoto H., Chuma S., Raducanu A., Nakatsuji N., Fassler R., Shinohara T. Homing of mouse spermatogonial stem cells to germline niche depends on b1-integrin.
  Cell Stem Cell3(5):533-542 (2008)
• Kanatsu-Shinohara M., Kato M., Takehashi M., Hirabayashi M., Shinohara T.
  Production of transgenic rats following lentiviral transduction and xenogeneic transplantation of spermatogonial stem cells.
  Biol. Reprod. 79(6):1121-1128 (2008)
• Kazuki Y., Hoshiya H., Kai Y., Abe S., Takiguchi M., Osaki M., Kawazoe S., Katoh M., Kanatsu-Shinohara M., Inoue K., Kajitani N., Yoshino T., Shirayoshi Y., Ogura A., Shinohara T., Barrett JC., Oshimura M. Correction of a genetic defect in multipotent germline stem cells using a human artificial chromosome.
  Gene Ther. 15(8):617-624 (2008)
• Kanatsu-Shinohara M., Muneto T., Lee J., Takenaka., Chuma S, Nakatsuji N., Horiuchi T., Shinohara T. Long-term culture of male germlinestem cells from hamster testes.
  Biol. Reprod.78(4):611-617 (2008)
• Kanatsu-Shinohara M., Lee J., Inoue K., Ogonuki N., Miki H., Toyokuni S.,Ikawa M., Nakamura T., Ogura A., Shinohara T. Pluripotency of a single spermatogonial stem cell in mice.
  Biol. Reprod. 78(4):681-687 (2008)
• Takehashi M., Kanatsu-Shinohara M., Miki H., Lee J., Kazuki Y., Inoue K., Ogonuki N., Toyokuni S., Oshimura M., Ogura A., Shinohara T. Production of knockout mice by gene targeting in multipotent germline stem cells.
  Dev. Biol. 312:344-352 (2007)
• Kanatu-Shinohara M., Shinohara T. Culture and genetic modification of mouse germline stem cells.
  Ann N Y Acad Sci. Dec;1120:59-71.(2007)
• Honda A., Hirose M., Hara K., Matoba S., Inoue K., Miki H., Hiura H., Kanatsu-Shinohara M., Kanai Y., Kono T., Shinohara T., Ogura A. Isolation, characterization, and in vitro differentiation of putative thecal stem cells.
  Proc. Natl. Acad. Sci. USA 104(30):12389-12394 (2007)
• Lee J., Kanatsu-Shinohara M., Inoue K., Ogonuki N., Miki H., Toyokuni S., Kimura T., Nakano T., Ogura A., Shinohara T. Akt mediates self-renewal division of mouse spermatogonial stem cells.
  Development 134(10):1853-1859 (2007)
• Baba S., Heike T., Ueda K., Iwasa T., Kaichi S., Hiraumi Y., Doi H., Yoshimoto M., Kanatsu-Shinohara M., Shinohra T., Nakahata T. Generation of cardiac and endothelial cells from neonatal mouse testis-derived multipotent cells.
  Stem cells 25(6):1375-83 (2007)
• Takehashi M., Kanatsu-Shinohara M., Inoue K., Ogonuki N., Miki H., Toyokuni S., Ogura A., Shinohara T. Adenovirus-mediated gene delivery into mouse spermatogonial stem cells.
  Proc. Natl. Acad. Sci. USA 104(8):2596-2601 (2007)
• Kurosaki H.,Kazuki Y., Hiratsuka M., Inoue T., Matsui Y., Wang CC., Kanatsu-Shinohara M., Shinohara T., Toda T., Oshimura M. A comparison study in the proteomic signatures of multipotent germline stem cells, embryonic stem cells, and germline stem cells.
  Biochem .Biophys. Res. sCommun. 353(2):259-267 (2007)
• Kanatsu-Shinohara M., Inoue K., Ogonuki N., Miki H., Yoshida S., Toyokuni S., Lee J., Ogura A., Shinohara T. Leukemia inhibitory factor enhances formation of germ cell colonies in neonatal mouse testis culture.
  Biol. Reprod. 76:55-62 (2007)
• Shinohara T., Kato M., Takehashi M., Lee J., Chuma S., Nakatsuji N., Kanatsu-Shinohara M., Hirabayashi M. Rat produced by interspecies spermatogonial transplantation in mice and in vitro microinsemination.
  Proc. Natl. Acad. Sci. USA 103:13624-8 (2006)
• Imamura M., Miura K., Iwabuchi K., Ichisaka T., Nakagawa M., Lee J., Kanatsu-Shinohara M., Shinohara T., Yamanaka S. Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells.
  BMC Dev. Biol. 6:34 (2006)
• Kanatsu-Shinohara M., Shinohara T.
  The germ of pluripotency.
  Nat. Biotechnol. 24:663-664 (2006)
• Fujino RS., Ishikawa Y., Tanaka K., Kanatsu-Shinohara M., Tamura K., Kogo H., Shinohara T., Hara T. Capillary morphogenesis gene(CMG)-1 is among the genes differentially expressed in mouse male germ line stem cells and embryonic stem cells.
  Mol. Reprod. Dev. 73:955-966 (2006)
• Kanatsu-Shinohara M. Ikawa M.l, Takehashi M., Ogonuki N., Miki H., Inoue K., Kazuki Y., Lee J., Toyokuni S., Oshimura M., Ogura A., Shinohara T. Production of knockout mice by random or targeted mutagenesis in spermatogonial stem cells.
  Proc. Natl. Acad. Sci. USA 103:8018-8023 (2006)
• Kanatsu-Shinohara M., Inoue K., Miki H., Ogonuki N., Takehashi M., Morimoto T., Ogura A., Shinohara T. Clonal origin of germ cell colonies after spermatogonial transplantation in mice.
  Biol. Reprod. 75:68-74 (2006)
• Kanatsu-Shinohara M., Inoue K., Lee J., Miki H., Ogonuki N., Toyokuni S., Ogura A., Shinohara T. Anchorage-independent growth of mouse male germline stem cells in vitro.
  Biol. Reprod. 74:522-529 (2006)
• Kanatsu-Shinohara M., Ogonuki N., Iwano T., Lee J., Kazuki Y., Inoue K., Miki H., Takehashi M., Toyokuni S., Shinkai Y., Oshimura M., Ishino F., Ogura A., Shinohara T. Genetic and epigenetic properties of mouse male germline stem cells during long-term culture.
  Development 132:4155-4163 (2005)
• Kanatsu-Shinohara M. Miki H., Inoue K., Ogonuki N., Toyokuni S., Ogura A., Shinohara T. Germline niche transplantation restores fertility in infertile mice.
  Hum. Reprod. 20:2376-2382 (2005)
• Kanatsu-Shinohara M., Miki H., Inoue K., Ogonuki N., Toyokuni S., Ogura A., Shinohara T. Long-term culture of mouse male germline stem cells under serum- or feeder-free conditions.
  Biol. Reprod. 72:985-991 (2005)
• Chuma S., Kanatsu-Shinohara M., Inoue K., Ogonuki N., Miki H., Toyokuni S., Hosokawa M., Nakatsuji N., Ogura A., Shinohara T. Spermatogenesis from epiblast and primordial germ cells following transplantation into postnatal mouse testis.
  Development 132:117-122 (2005)
• Kanatsu-Shinohara M., Toyokuni, S., Shinohara, T. Genetic selection of mouse male germline stem cells in vitro: offspring from single stem cells.
  Biol. Reprod. 72:236-240 (2005)
• Kanatsu-Shinohara M., Inoue K., Lee J., Yoshimoto M., Ogonuki N., Miki H., Baba S., Kato T., Kazuki Y., Toyokuni S., Toyoshima M., Niwa O., Oshimura M., Heike T., Nakahata T., Ishino F., Ogura A., Shinohara T. Generation of pluripotent stem cells from neonatal mouse testis.
  Cell 119:1001-1012 (2004)
• Kanatsu-Shinohara M., Toyokuni S., Shinohara T. Transgenic mice produced by retroviral transduction of male germline stem cells in vivo.
  Biol. Reprod. 71:1202-1207 (2004)
• Kanatsu-Shinohara M., Morimoto T., Toyokuni S., Shinohara T. Regulation of mouse spermatogonial stem cell self-renewing division by the pituitary gland.
  Biol. Reprod. 70:1731-1737 (2004)
• Kanatsu-Shinohara M., Toyokuni S., Shinohara T. CD9 is a surface marker on mouse and rat male germline stem cells.
  Biol. Reprod. 70:70-75 (2004)
• Kanatsu-Shinohara M., Ogonuki N., Inoue K., Ogura A., Toyokuni S., Shinohara T. Restoration of fertility in infertile mice by transplantation of cryopreserved male germline stem cells.
  Hum. Reprod. 18:2660-2667 (2003)
• Yoshimoto M., Shinohara T., Heike T., Shiota M., Kanatsu-Shinohara M., Nakahata T. Direct visualization of transplanted hematopoietic cell reconstitution in intact mouse organs indicates the presence of a niche.
  Exp. Hematol. 31:733-40 (2003)
• Kanatsu-Shinohara M., Ogonuki N., Inoue K., Miki H., Ogura A., Toyokuni S., Shinohara T. Long-term proliferation in culture and germline transmission of mouse male germline stem cells.
  Biol. Reprod. 69:612-616 (2003)
• Kanatsu-Shinohara M., Toyokuni S., Morimoto T., Matsui S., Honjo T., Shinohara T. Functional assessment of self-renewal activity of male germline stem cells following cytotoxic damage and serial transplantation.
  Biol. Reprod. 68:1801-1807 (2003)
• Kanatsu-Shinohara M., Ogonuki N., Inoue K., Ogura A., Toyokuni S., Honjo T., Shinohara T. Allogeneic offspring produced by male germ line stem cell transplantation into infertile mouse testis.
  Biol. Reprod. 68:167-173 (2003)
• Shinohara T., Inoue K., Ogonuki N., Kanatsu-Shinohara M., Miki H., Nakata K., Kurome M., Nagashima H., Toyokuni S., Kogishi K., Honjo T., Ogura A. Birth of offspring following transplantation of cryopreserved immature testicular pieces and in vitro microinsemination.
  Hum. Reprod17:3039-3034 (2002)
• Kanatsu-Shinohara M. Ogura A., Ikegawa M., Inoue K., Ogonuki N., Tashiro K., Toyokuni S., Honjo T., Shinohara T. Adenovirus-mediated gene delivery and in vitro microinsemination produce offspring from infertile male mice.
  Proc. Natl. Acad. Sci. USA 99:1383-1388 (2002)
• Shinohara T., Orwig KE., Brinster RL. Remodeling of the postnatal mouse testis is accompanied by dramatic changes in stem cell number and niche accessibility.
  Proc. Natl. Acad. Sci. USA 98(11):6186-91 (2001)
• Shinohara T., Orwig KE., Avarbock MR., Brinster RL. Spermatogonial stem cell enrichment by multiparameter selection of mouse testis cells.
  Proc. Natl. Acad. Sci. USA 97(15):8346-51 (2000)
• Shinohara T., Avarbock MR., Brinster RL.
  Functional analysis of spermatogonial stem cells in Steel and cryptorchid infertile mouse models.
  Dev. Biol. 220(2):401-11 (2000)
• Shinohara T1, Avarbock MR, Brinster RL.
  beta1- and alpha6-integrin are surface markers on mouse spermatogonial stem cells.
  Proc. Natl. Acad. Sci. USA 96:5504-5509 (1999).
• Shinohara, T., Honjo, T.
  Studies in vitro on the mechanism of the epithelial/mesenchymal interaction in the early fetal thymus.
  Eur. J. Immunol.27:522-529 (1997).
• Shinohara, T., Honjo, T.
  Epidermal growth factor can replace thymic mesenchyme in induction of embryonic thymus morphogenesis in vitro.
  Eur. J. Immunol.26:747-752 (1996).
• Shirozu, M., Nakano, T., Inazawa, J., Tashiro, K., Tada, H., Shinohara, T., Honjo, T.
  Structure and chromosomal localization of the human stromal cell-derived factor 1 (SDF1) gene.
  Genomics28:495-500 (1995).
• Shinohara, T., Taniwaki, M., Ishida, Y., Kawaichi, M., Honjo, T.
  Structure and chromosomal localization of the human PD-1 gene (PDCD1).
  Genomics23:704-706 (1994).